Cenpa^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cenpa^{tm1c(EUCOMM)Wtsi}
• Name: centromere protein A; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5896608
• Synonyms: CENP-A floxed
• Gene: Cenpa Location: Chr5:30824214-30832181 bp, + strand Genetic Position: Chr5, 16.76 cM
• Alliance: Cenpa^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cenpa gene page

Mutation origin

• Mutant Cell Line: EPD0445_6_E07
• Germline Transmission: Earliest citation of germline transmission: J:240675
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30829333 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 30832460. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:240675)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cenpa Mutation: 15 strains or lines available

References

• Original: J:240675 Shirakawa J, et al., Insulin Signaling Regulates the FoxM1/PLK1/CENP-A Pathway to Promote Adaptive Pancreatic beta Cell Proliferation. Cell Metab. 2017 Apr 04;25(4):868-882.e5
• All: 3 reference(s)