Tnpo3^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tnpo3^{tm1d(KOMP)Wtsi}
• Name: transportin 3; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5888906
• Gene: Tnpo3 Location: Chr6:29540826-29609886 bp, - strand Genetic Position: Chr6, 12.36 cM, cytoband A3
• Alliance: Tnpo3^{tm1d(KOMP)Wtsi} page
• IMPC: Tnpo3 gene page

Mutation origin

• Mutant Cell Line: EPD0318_3_G02
• Germline Transmission: Earliest citation of germline transmission: J:240645
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 29578236 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 29579082. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:240645)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnpo3 Mutation: 78 strains or lines available

References

• Original: J:240645 Iwanami N, et al., Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development. Cell Rep. 2016 Nov 22;17(9):2259-2270
• All: 2 reference(s)