Gper1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gper1^{tm1c(KOMP)Wtsi}
• Name: G protein-coupled estrogen receptor 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5888889
• Synonyms: GPER^LoxP
• Gene: Gper1 Location: Chr5:139408906-139413555 bp, + strand Genetic Position: Chr5, 78.58 cM, cytoband G1
• Alliance: Gper1^{tm1c(KOMP)Wtsi} page
• IMPC: Gper1 gene page

Mutation origin

• Mutant Cell Line: EPD0334_2_H06
• Germline Transmission: Earliest citation of germline transmission: J:240653
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 139410951 of Chromosome 5 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 139412865. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 3. (J:240653)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gper1 Mutation: 27 strains or lines available

References

• Original: J:240653 Wang H, et al., Cardiomyocyte-specific deletion of the G protein-coupled estrogen receptor (GPER) leads to left ventricular dysfunction and adverse remodeling: A sex-specific gene profiling analysis. Biochim Biophys Acta. 2017 Aug;1863(8):1870-1882
• All: 3 reference(s)