Satb2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Satb2^{tm1c(KOMP)Wtsi}
• Name: special AT-rich sequence binding protein 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5888867
• Synonyms: Satb2^flox, Satb2^flox(KOMP)
• Gene: Satb2 Location: Chr1:56833140-57017809 bp, - strand Genetic Position: Chr1, 28.72 cM
• Alliance: Satb2^{tm1c(KOMP)Wtsi} page
• IMPC: Satb2 gene page

Mutation origin

• Mutant Cell Lines: EPD0098_3_G07, EPD0098_3_H05
• Germline Transmission: Earliest citation of germline transmission: J:226916
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 56937921 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 56938731. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:226916)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Satb2 Mutation: 46 strains or lines available

References

• Original: J:226916 McKenna WL, et al., Mutual regulation between Satb2 and Fezf2 promotes subcerebral projection neuron identity in the developing cerebral cortex. Proc Natl Acad Sci U S A. 2015 Sep 15;112(37):11702-7
• All: 12 reference(s)