Uhrf1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Uhrf1^{tm1c(EUCOMM)Wtsi}
• Name: ubiquitin-like, containing PHD and RING finger domains, 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5888866
• Synonyms: Uhrf1^fl
• Gene: Uhrf1 Location: Chr17:56610405-56630486 bp, + strand Genetic Position: Chr17, 29.26 cM, cytoband D-E1
• Alliance: Uhrf1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Uhrf1 gene page

Mutation origin

• Mutant Cell Line: EPD0052_1_G08
• Germline Transmission: Earliest citation of germline transmission: J:234945
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 56617252 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56618186. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:234945)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Uhrf1 Mutation: 53 strains or lines available

References

• Original: J:234945 Cui Y, et al., Uhrf1 Controls iNKT Cell Survival and Differentiation through the Akt-mTOR Axis. Cell Rep. 2016 Apr 12;15(2):256-63
• All: 8 reference(s)