Paxx^tm1.1Shzh
Targeted Allele Detail

Summary

• Symbol: Paxx^tm1.1Shzh
• Name: non-homologous end joining factor; targeted mutation 1.1, Shan Zha
• MGI ID: MGI:5888857
• Synonyms: Paxx^-
• Gene: Paxx Location: Chr2:25345153-25351106 bp, - strand Genetic Position: Chr2, 17.27 cM
• Alliance: Paxx^tm1.1Shzh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238544
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: All coding exons of the gene and part of the non-coding exon 1 were replaced by a Neomycin resistance (NeoR) cassette flanked by frt sequences. Chimeras were bred with mice expressing FLIPase constitutively to remove the Neo cassette and generate the recombined allele. Western blotting validated the absence of PAXX protein in murine embryonic fibroblasts derived from homozygous mutant mice. (J:238544)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Paxx Mutation: 9 strains or lines available

References

• Original: J:238544 Liu X, et al., PAXX promotes KU accumulation at DNA breaks and is essential for end-joining in XLF-deficient mice. Nat Commun. 2017 Jan 04;8:13816
• All: 1 reference(s)