Smyd2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Smyd2^{tm1c(KOMP)Wtsi}
• Name: SET and MYND domain containing 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5883837
• Synonyms: Smyd2^flox
• Gene: Smyd2 Location: Chr1:189612689-189654560 bp, - strand Genetic Position: Chr1, 95.03 cM, cytoband H6
• Alliance: Smyd2^{tm1c(KOMP)Wtsi} page
• IMPC: Smyd2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240508
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt2 cassette was inserted at position 189641827 of Chromosome 1 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 2 at position 189642549. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the exon 2. (J:240508)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smyd2 Mutation: 140 strains or lines available

References

• Original: J:240508 Bagislar S, et al., Smyd2 is a Myc-regulated gene critical for MLL-AF9 induced leukemogenesis. Oncotarget. 2016 Oct 11;7(41):66398-66415
• All: 2 reference(s)