Tg(Hnrnpa2b1-rtTA2S*M2)4Jstr
Transgene Detail

Summary

• Symbol: Tg(Hnrnpa2b1-rtTA2S*M2)4Jstr
• Name: transgene insertion 4, John Strouboulis
• MGI ID: MGI:5882798
• Synonyms: hnRNPA2B1-rtTA2S-M2, HNRPA2B1/rtTA2S-M2
• Transgene: Tg(Hnrnpa2b1-rtTA2S*M2)4Jstr Location: unknown
• Alliance: Tg(Hnrnpa2b1-rtTA2S*M2)4Jstr page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: Mice bearing this transgene express the highly doxycycline-sensitive rtTA2S-M2 reverse tetracycline transactivator (tet-on) variant under control of the human HNRNPA2B1 promoter. An ~8-kb human genomic DNA fragment was used that spans the CpG island containing the divergently transcribed promoters of the ubiquitously expressed housekeeping genes HNRNPA2B1 and CBX3. Transgenic lines were established from seven founder mice. Line 9 (Tg(Hnrnpa2b1-rtTA2S*M2)9Jstr), exhibiting the highest level of expression detected by northern blot analysis in all tissues examined, was expanded for further study. Transgenic founder 2 had two integration sites, which separated upon breeding to generate lines 2a and 2b; both express the transgene weakly in some tissues. Lines 1, 4, 6 and 7 express low but detectable levels. (J:128742)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:128742 Katsantoni EZ, et al., Ubiquitous expression of the rtTA2S-M2 inducible system in transgenic mice driven by the human hnRNPA2B1/CBX3 CpG island. BMC Dev Biol. 2007;7:108
• All: 1 reference(s)