Sdc4^em#Qin
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sdc4^em#Qin
• Name: syndecan 4; endonuclease-mediated mutation, Qinmiao Sun
• MGI ID: MGI:5882687
• Gene: Sdc4 Location: Chr2:164266167-164285512 bp, - strand Genetic Position: Chr2, 85.16 cM
• Alliance: Sdc4^em#Qin page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The first exon of the gene was targeted using the CRISPR/Cas9 system using two sgRNAs (5'-GCAGCAGCAGCGGCGCAAGC-3' and 5'-AGCATCTTCGTCGTCGGGGA-3'). This resulted in three mouse lines with different mutations: 1 bp deletion, 10 bp deletion and 1 bp insertion. All three mutations cause reading frameshifts and premature translation termination. Immunoblots confirmed absence of peptides produced from this allele. It is not specified whether a pool of the three mouse lines was used or a specific single line. (J:239894)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdc4 Mutation: 33 strains or lines available

References

• Original: J:239894 Lin W, et al., Syndecan-4 negatively regulates antiviral signalling by mediating RIG-I deubiquitination via CYLD. Nat Commun. 2016 Jun 09;7:11848
• All: 1 reference(s)