Ric8a^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ric8a^{tm1c(KOMP)Wtsi}
• Name: RIC8 guanine nucleotide exchange factor A; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5882612
• Synonyms: Ric-8A^Flox
• Gene: Ric8a Location: Chr7:140437310-140443644 bp, + strand Genetic Position: Chr7, 86.06 cM
• Alliance: Ric8a^{tm1c(KOMP)Wtsi} page
• IMPC: Ric8a gene page

Mutation origin

• Mutant Cell Line: EPD0394_4_A01
• Germline Transmission: Earliest citation of germline transmission: J:236020
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 140439024 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 140439767. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:236020)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ric8a Mutation: 29 strains or lines available

References

• Original: J:236020 Patel BR, et al., Ric-8A gene deletion or phorbol ester suppresses tumorigenesis in a mouse model of GNAQ(Q209L)-driven melanoma. Oncogenesis. 2016 Jun 27;5(6):e236
• All: 1 reference(s)