Tg(Thy1-mseCFP/atpc*/mVenus*)AJhi
Transgene Detail

Summary

• Symbol: Tg(Thy1-mseCFP/atpc*/mVenus*)AJhi
• Name: transgene insertion A, Johannes Hirrlinger
• MGI ID: MGI:5882597
• Synonyms: AT1.03^YEMK, Thy1.2-ATeam1.03YEMK, ThyAT
• Transgene: Tg(Thy1-mseCFP/atpc*/mVenus*)AJhi Location: unknown
• Alliance: Tg(Thy1-mseCFP/atpc*/mVenus*)AJhi page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains a mouse promoter Thy1.2 (Thy^b) driving neuronal expression of a FRET-based ATP indicator (J:240165) composed of a variant of cyan fluorescent protein (mseCFP) linked with circularly permuted mVenus having the 173rd amino acid as its N terminus (cp173-mVenus) to either the N- or C terminus of the epsilon subunit of the Bacillus subtilis FoF1-ATP synthase Adenosine 5-Triphosphate (atpc). Four hydrophobic amino acid residues (Val-9, Leu-42, Phe-67, and Leu-78) of the epsilon subunit were replaced by hydrophilic residues to retard incorporation into the FoF1 and reduce nonspecific hydrophobic interaction with other proteins. In addition, M60Y, G131E, K132M and A133K mutations were introduced. This sensor is known as ATeam (adenosine 5-Triphosphate indicator based on Epsilon subunit for Analytical Measurements) 1.03YEMK (ATeam1.03YEMK) and can be used for measuring intracellular ATP levels in the millimolar range. Line A has 21 copies of the transgene. (J:240165, J:240525)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:240525 Trevisiol A, et al., Monitoring ATP dynamics in electrically active white matter tracts. Elife. 2017 Apr 17;6:e24241
• All: 7 reference(s)