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Smc5tm1d(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Smc5tm1d(KOMP)Wtsi
Name: structural maintenance of chromosomes 5; targeted mutation 1d, Wellcome Trust Sanger Institute
MGI ID: MGI:5882566
Synonyms: Smc5del
Gene: Smc5  Location: Chr19:23206451-23273897 bp, - strand  Genetic Position: Chr19, 18.04 cM
Mutation
origin
Mutant Cell Line:  EPD0395_1_F05
Germline Transmission:  Earliest citation of germline transmission: J:240155
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 23264250 of Chromosome 19 upstream of exon 4 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 23263369. Exon 4 is thus flanked by loxP sites. A null/knockout allele was created by flp- and cre-mediated recombination to remove the lacZ sequence, neo cassette and the floxed exon 4. (J:240155)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Smc5 Mutation:  20 strains or lines available
References
Original:  J:240155 Pryzhkova MV, et al., Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression. J Cell Sci. 2016 Apr 15;129(8):1619-34
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/03/2020
MGI 6.15
The Jackson Laboratory