Asic3^tm2.1Cibms
Targeted Allele Detail

Summary

• Symbol: Asic3^tm2.1Cibms
• Name: acid-sensing ion channel 3; targeted mutation 2.1, Chih-Cheng Chen
• MGI ID: MGI:5882389
• Synonyms: Asic3delta^EGFPf
• Gene: Asic3 Location: Chr5:24618449-24622836 bp, + strand Genetic Position: Chr5, 11.72 cM
• Alliance: Asic3^tm2.1Cibms page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239934
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The sequence coding for exon 1, intron 1 and most of exon 2 was replaced with the following: an EGFP reporter gene cassette, an FRT site flanked neomycin resistance gene cassette and the WGA (wheat germ agglutinin) cDNA. LoxP sites were placed upstream of the EGFP cassette and between the neo cassette and the WGA cDNA. The neo cassette was removed through subsequent flp-mediated recombination. RT-qPCR showed barely detectable levels of target gene transcripts in mice homozygous for the allele and abundant expression of the EGFP knock-in gene. (J:239934)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Asic3 Mutation: 28 strains or lines available

References

• Original: J:239934 Lin SH, et al., Evidence for the involvement of ASIC3 in sensory mechanotransduction in proprioceptors. Nat Commun. 2016 May 10;7:11460
• All: 2 reference(s)