Asic3^tm1.2Cibms
Targeted Allele Detail

Summary

• Symbol: Asic3^tm1.2Cibms
• Name: acid-sensing ion channel 3; targeted mutation 1.2, Chih-Cheng Chen
• MGI ID: MGI:5882386
• Gene: Asic3 Location: Chr5:24618449-24622836 bp, + strand Genetic Position: Chr5, 11.72 cM
• Alliance: Asic3^tm1.2Cibms page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239934
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 1. mRNA expression levels were studied through RT-PCR and determined that the neo cassette interfered with the transcription of the allele. Therefore the neo cassette was removed through subsequent flp-mediated recombination. Exon 1 was deleted by subsequent cre-mediated recombination, creating a null allele. RT-PCR confirmed depletion of transcripts from this allele. (J:239934)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Asic3 Mutation: 28 strains or lines available

References

• Original: J:239934 Lin SH, et al., Evidence for the involvement of ASIC3 in sensory mechanotransduction in proprioceptors. Nat Commun. 2016 May 10;7:11460
• All: 3 reference(s)