Gsdmd^em#Gne
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gsdmd^em#Gne
• Name: gasdermin D; endonuclease-mediated mutation, Genentech
• MGI ID: MGI:5882077
• Gene: Gsdmd Location: Chr15:75734176-75739257 bp, + strand Genetic Position: Chr15, 35.0 cM, cytoband D3-E1
• Alliance: Gsdmd^em#Gne page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cytoplasmic injection of Cas9 mRNA and sgRNAs (5'-GAGAAGGGAAAATTTCTGG-3' and 5'-AGGGCAGAGTGATGTTGTC-3') into zygotes resulted in compound heterozygous F1 mice (from F0 founder crosses) and homozygous F2 mice (from F1 crosses). Mutations varied from 1-5 bp insertions to 1-1632 bp deletions. A total of 12 different alleles were generated. (J:226780)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gsdmd Mutation: 33 strains or lines available

References

• Original: J:226780 Kayagaki N, et al., Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling. Nature. 2015 Oct 29;526(7575):666-71
• All: 9 reference(s)