Insr^tm1.1Shlm
Targeted Allele Detail

Summary

• Symbol: Insr^tm1.1Shlm
• Name: insulin receptor; targeted mutation 1.1, Gerald Shulman
• MGI ID: MGI:5881945
• Synonyms: Insr^T1150A
• Gene: Insr Location: Chr8:3200922-3329649 bp, - strand Genetic Position: Chr8, 1.82 cM
• Alliance: Insr^tm1.1Shlm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239615
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6J-A^w-J/J

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Exon 19 was replaced with a copy containing an engineered mutation changing Thr1150 codon ACA to alanine codon GCC. This mutation prevents phosphorylation of the peptide at that residue and creates a kinase dead version of the peptide. It is the equivalent of a recombinant engineered Thr1160Ala mutation in human thought to protect the peptide from inhibition by PRKCE. The FRT site flanked neomycin resistance gene cassette that was inserted into intron 18 was removed through flp-mediated recombination. (J:239615)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Insr Mutation: 95 strains or lines available

Notes

ES cell line = B6Aw6

References

• Original: J:239615 Petersen MC, et al., Insulin receptor Thr1160 phosphorylation mediates lipid-induced hepatic insulin resistance. J Clin Invest. 2016 Nov 01;126(11):4361-4371
• All: 2 reference(s)