Apln^tm2Ntak
Targeted Allele Detail

Summary

• Symbol: Apln^tm2Ntak
• Name: apelin; targeted mutation 2, Nobuyuki Takakura
• MGI ID: MGI:5829356
• Synonyms: apelin flox(neo,ex2)
• Gene: Apln Location: ChrX:47114023-47123730 bp, - strand Genetic Position: ChrX, 25.48 cM, cytoband A3.2
• Alliance: Apln^tm2Ntak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239279
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 47117021 of Chromosome X upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 47117722. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:239279)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apln Mutation: 12 strains or lines available

References

• Original: J:239279 Kidoya H, et al., APJ Regulates Parallel Alignment of Arteries and Veins in the Skin. Dev Cell. 2015 May 04;33(3):247-59
• All: 1 reference(s)