Cstb^tm2Yah
Targeted Allele Detail

Summary

• Symbol: Cstb^tm2Yah
• Name: cystatin B; targeted mutation 2, Yann Herault
• MGI ID: MGI:5828767
• Synonyms: CSTB^5RT, Cstb^tm1Yah
• Gene: Cstb Location: Chr10:78261504-78263456 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Cstb^tm2Yah page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181132
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Duplication
• Mutation details: A tandem duplication of the gene resulted from integration of a vector containing a neomycin selection cassette, a loxP site, the 5' end of the Hprt gene, and a homology region including all three exons of the targeted gene. The two copies of the gene are separated in the mouse chromosome by the vector sequence. Quantitative real-time PCR of total RNA extracts of liver and brain demonstrated ~2-fold greater transcript levels, relative to those of wild-type mice, in both tissues of mice with three gene copies and ~3-fold increase in mice with four copies. (J:181132)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cstb Mutation: 12 strains or lines available

References

• Original: J:181132 Brault V, et al., Characterization of PTZ-induced seizure susceptibility in a down syndrome mouse model that overexpresses CSTB. PLoS One. 2011;6(11):e27845
• All: 3 reference(s)