Ryr2^tm4.1Swch
Targeted Allele Detail

Summary

• Symbol: Ryr2^tm4.1Swch
• Name: ryanodine receptor 2, cardiac; targeted mutation 4.1, S R Wayne Chen
• MGI ID: MGI:5828593
• Synonyms: GFP-RyR2
• Gene: Ryr2 Location: Chr13:11567988-12121831 bp, - strand Genetic Position: Chr13, 4.38 cM, cytoband A1-A2
• Alliance: Ryr2^tm4.1Swch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239358
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An FRT site flanked neomycin resistance gene cassette was inserted into intron 31. A green fluorescent protein (GFP) gene was inserted into exon 31, in-frame after the Thr1366 codon in Divergent Region II. To minimise steric hindrance, the GFP gene was flanked both sides by 10-codon linkers rich in glycine codons. The neo cassette was subsequently removed through flp-mediated recombination. (J:239358)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ryr2 Mutation: 330 strains or lines available

References

• Original: J:239358 Hiess F, et al., Distribution and Function of Cardiac Ryanodine Receptor Clusters in Live Ventricular Myocytes. J Biol Chem. 2015 Aug 14;290(33):20477-87
• All: 4 reference(s)