Erc1^tm1Ksak
Targeted Allele Detail

Summary

• Symbol: Erc1^tm1Ksak
• Name: ELKS/RAB6-interacting/CAST family member 1; targeted mutation 1, Kenji Sakimura
• MGI ID: MGI:5827582
• Synonyms: ELKS-S55A, ELKS^S55A
• Gene: Erc1 Location: Chr6:119547757-119825128 bp, - strand Genetic Position: Chr6, 56.86 cM, cytoband F1
• Alliance: Erc1^tm1Ksak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238996
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An FRT site flanked neomycin resistance gene cassette was inserted into intron 1. Exon 1 was targeted with two codon changes: a silent mutation of L54 (CTT to CTG) and an S55A (TCA to GCC) mutation. These mutations create a diagnostic MscI restriction site and changes serine 55 to an alanine, thus preventing phosphorylation of the peptide at that position. (J:238996)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Erc1 Mutation: 61 strains or lines available

References

• Original: J:238996 Mochida S, et al., SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression. Cell Rep. 2016 Sep 13;16(11):2901-13
• All: 1 reference(s)