Sting1^em1Tsse
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sting1^em1Tsse
• Name: stimulator of interferon response cGAMP interactor 1; endonuclease-mediated mutation 1, Tsukasa Seya
• MGI ID: MGI:5827553
• Synonyms: Tmem173^Cr5
• Gene: Sting1 Location: Chr18:35866732-35873607 bp, - strand Genetic Position: Chr18, 19.23 cM, cytoband B3
• Alliance: Sting1^em1Tsse page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 3 was targeted with the CRISPR/Cas9 system. The sgRNA sequence was 5'-CACCTAGCCTCGCACGAActt-3' (recognition sequence in upper case) and the experiment resulted in a 5 bp deletion (CTCGC) in the middle of the recognition sequence. The resulting frameshift introduces a premature stop codon soon after, knocking the gene out. Western blots confirmed the absence of peptides translated from this allele in homozygous mice. (J:239003)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sting1 Mutation: 45 strains or lines available

References

• Original: J:239003 Takashima K, et al., STING in tumor and host cells cooperatively work for NK cell-mediated tumor growth retardation. Biochem Biophys Res Commun. 2016 Sep 30;478(4):1764-71
• All: 6 reference(s)