Zfy2^em3Asah
Endonuclease-mediated Allele Detail

Summary

• Symbol: Zfy2^em3Asah
• Name: zinc finger protein 2, Y-linked; endonuclease-mediated mutation 3, Hiroshi Asahara
• MGI ID: MGI:5824902
• Gene: Zfy2 Location: ChrY:2106175-2170409 bp, - strand Genetic Position: ChrY, cytoband p
• Alliance: Zfy2^em3Asah page

Mutation origin

• Strain of Origin: B6D2F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 4 was targed for modification using the CRISPR/Cas9 system. The sgRNA target sequence was 5'-GAAGCAGTCTTAGATTCCAGtgg-3' (PAM sequence in lower case). As this gRNA targets identical sequence in this gene and in Zfy1, the procedure yielded single knock-outs (one founder for each gene) as well as double knock-outs (10 founders). Genotyping of this knock-out allele found in double knock-out founder #2 revealed 6 and 4 bp insertions (2 bp apart) in the target sequence. (J:238696)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfy2 Mutation: 28 strains or lines available

References

• Original: J:238696 Nakasuji T, et al., Complementary Critical Functions of Zfy1 and Zfy2 in Mouse Spermatogenesis and Reproduction. PLoS Genet. 2017 Jan;13(1):e1006578
• All: 1 reference(s)