Klk5^tm1Rase
Targeted Allele Detail

Summary

• Symbol: Klk5^tm1Rase
• Name: kallikrein related-peptidase 5; targeted mutation 1, Radislav Sedlacek
• MGI ID: MGI:5824533
• Gene: Klk5 Location: Chr7:43491395-43500605 bp, + strand Genetic Position: Chr7, 28.3 cM
• Alliance: Klk5^tm1Rase page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238698
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This 'knockout first' allele was created using the KOMP targeting vector for this gene (PRPGS00082_A_A10). It inserts a beta galactosidase (lacZ) reporter gene trap and a neomycin resistance gene cassette into intron 1 and flanks exon 2 with loxP sites. The lacZ-neo double cassette is flanked by FRT sites and the two cassettes are separated by a loxP site. Insertion of the lacZ gene trap (which starts with a strong splice acceptor site) promotes splicing of exon 1 of the target gene to the lacZ gene, thus disrupting normal transcription and creating a null allele. (J:238698)

Expression

In Mice Carrying this Mutation:

51 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klk5 Mutation: 23 strains or lines available

References

• Original: J:238698 Kasparek P, et al., KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype. PLoS Genet. 2017 Jan;13(1):e1006566
• All: 1 reference(s)