Tg(KRT14-Notch*/ESR1)CA4Fmw
Transgene Detail

Summary

• Symbol: Tg(KRT14-Notch*/ESR1)CA4Fmw
• Name: transgene insertion CA4, Fiona M Watt
• MGI ID: MGI:5824128
• Synonyms: K14N^ICDdeltaOPER, K14NICDER, line 5199.21A,B+C, line CA4
• Transgene: Tg(KRT14-Notch*/ESR1)CA4Fmw Location: unknown
• Alliance: Tg(KRT14-Notch*/ESR1)CA4Fmw page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)F1

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Tg(KRT14-Notch*/ESR1)CA4Fmw expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Notch1 (MGI:97363)	expresses amino acids 1751-2290 of the intracellular domain

• Mutation details: The transgene consists of a construct that expresses amino acids 1751-2290 of the mouse Notch1 intracellular domain (NICDOP) with an N-terminal FLAG sequence and a modified estrogen receptor (ER) fused in frame at the C terminus. Expression of the construct is driven by a keratin 14 promoter cassette. Founder lines 5199.15A,B+C (CA3, low copy number) and 5199.21A,B+C (CA4, contains 21 copies of the transgene at high copy number) were established; both lines had a similar phenotype, the severity correlating with copy number. Line CA4 was studied further. (J:115280, J:142873)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:115280 Estrach S, et al., Jagged 1 is a {beta}-catenin target gene required for ectopic hair follicle formation in adult epidermis. Development. 2006 Nov;133(22):4427-38
• All: 4 reference(s)