Firre^tm1.1Rinn
Targeted Allele Detail

Summary

• Symbol: Firre^tm1.1Rinn
• Name: functional intergenic repeating RNA element; targeted mutation 1.1, John Rinn
• MGI ID: MGI:5824118
• Gene: Firre Location: ChrX:49651997-49724198 bp, - strand Genetic Position: ChrX, 26.53 cM
• Alliance: Firre^tm1.1Rinn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261345
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP-flanked Neomycin selection cassette is inserted downstream of the gene, and a loxP-flanked hygromycin cassette is inserted upstream of the gene. Cre-mediated recombination removed the both floxed cassettes, the promoter and 81.8 kb of Firre sequence. No gene product is detected by in situ hybridization of whole mount E11.5 homozygous embryos. (J:261345)

Expression

In Mice Carrying this Mutation:

1 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Firre Mutation: 14 strains or lines available

References

• Original: J:261345 Barutcu AR, et al., A TAD boundary is preserved upon deletion of the CTCF-rich Firre locus. Nat Commun. 2018 Apr 13;9(1):1444
• All: 3 reference(s)