Hsd11b1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hsd11b1^{tm1c(KOMP)Wtsi}
• Name: hydroxysteroid 11-beta dehydrogenase 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5819106
• Synonyms: Hsd11b1^flox
• Gene: Hsd11b1 Location: Chr1:192903917-192946345 bp, - strand Genetic Position: Chr1, 97.67 cM
• Alliance: Hsd11b1^{tm1c(KOMP)Wtsi} page
• IMPC: Hsd11b1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236052
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 192922248 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 192923393. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:236052)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsd11b1 Mutation: 29 strains or lines available

References

• Original: J:236052 Itoi-Ochi S, et al., Local corticosterone activation by 11beta-hydroxysteroid dehydrogenase 1 in keratinocytes: the role in narrow-band UVB-induced dermatitis. Dermatoendocrinol. 2016 Jan-Dec;8(1):e1119958
• All: 3 reference(s)