Prnp^em1Aag
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prnp^em1Aag
• Name: prion protein; endonuclease-mediated mutation 1, Adriano Aguzzi
• MGI ID: MGI:5819099
• Synonyms: Prnp^ZH3
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^em1Aag page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A TALEN pair was used to target a site in close proximity to the start codon. An 8-bp deletion was identified in exon 3. The frame shift introduced a premature stop codon in the sequence coding for the secretory signal peptide. Homozygous mice show no detectable protein expression in central nervous system (CNS) tissues, as assessed by Western blotting. (J:233015)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 143 strains or lines available

References

• Original: J:233015 Nuvolone M, et al., Strictly co-isogenic C57BL/6J-Prnp-/- mice: A rigorous resource for prion science. J Exp Med. 2016 Mar 7;213(3):313-27
• All: 13 reference(s)