Cdkn1a^tm1.1Wolf
Targeted Allele Detail

Summary

• Symbol: Cdkn1a^tm1.1Wolf
• Name: cyclin dependent kinase inhibitor 1A; targeted mutation 1.1, C Roland Wolf
• MGI ID: MGI:5818958
• Gene: Cdkn1a Location: Chr17:29309953-29319696 bp, + strand Genetic Position: Chr17, 15.12 cM
• Alliance: Cdkn1a^tm1.1Wolf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237855
• Parent Cell Line: ART B6-3, B6-3 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A beta galactosidase (lacZ) and a floxed luciferase reporter gene cassette were inserted between the penultimate codon and the stop codon in exon 3 and an FRT site flanked puromycin resistance gene cassette was inserted downstream of exon 3. The reporter gene sequences were preceded by sequence coding for self-cleaving T2A peptide. The puro cassette was removed through flp-mediated recombination, yielding a dual reporter allele. Western blots showed expression of the gene from this allele to be considerably lower than from the wild-type allele. (J:237855)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdkn1a Mutation: 63 strains or lines available

References

• Original: J:237855 McMahon M, et al., Olaparib, Monotherapy or with Ionizing Radiation, Exacerbates DNA Damage in Normal Tissues: Insights from a New p21 Reporter Mouse. Mol Cancer Res. 2016 Dec;14(12):1195-1203
• All: 1 reference(s)