Kit^tm1.2Ccai
Targeted Allele Detail

Summary

• Symbol: Kit^tm1.2Ccai
• Name: Kit proto-oncogene receptor tyrosine kinase; targeted mutation 1.2, Chen-Leng Cai
• MGI ID: MGI:5818517
• Synonyms: c-kit^H2B-tdTomato
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^tm1.2Ccai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227907
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A LoxP-4XPolyA-LoxP-H2B-tdTomate-FRT-Neo-FRT cassette was targeted to the start codon of kit locus with disruption of endogenous ATG by homologous recombination in ES cells. Flp-mediated recombination removed the neo selection cassette, leaving a single FRT site behind. Resulting mouse line was crossed with Protamine-Cre mice to remove 4xPloyA stop cassette. (J:227907)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 177 strains or lines available

References

• Original: J:227907 Sultana N, et al., Resident c-kit(+) cells in the heart are not cardiac stem cells. Nat Commun. 2015;6:8701
• All: 3 reference(s)