About   Help   FAQ
Gt(ROSA)26Sortm1(CAG-HIST1H2BJ/mCherry,-EGFP/Rpl10a)Evdr
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm1(CAG-HIST1H2BJ/mCherry,-EGFP/Rpl10a)Evdr
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Evan Rosen
MGI ID: MGI:5817798
Synonyms: H2B-TRAP
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
 
Mutation detailsThe targeting vector was designed with (from 5' to 3') with a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a FRT site, a loxP-flanked STOP, the H2B-mCherry fusion protein (coding sequence for the human histone 1 H2bj gene [HIST1H2BJ] fused in-frame to the N-terminus of a monomeric red fluorescent protein sequence [mCherry]), a 2A peptide, the EGFP-L10a fusion protein (an enhanced green fluorescent protein gene [EGFP] fused in-frame to the N-terminus of a cDNA sequence encoding the mouse 60S ribosomal subunit L10a [Rpl10a]), WPRE,a polyA signal, an AttB site, PGK-FRT-Neo-PGK polyA), and an AttP site. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:101977)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  534 strains or lines available
References
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
07/29/2020
MGI 6.15
The Jackson Laboratory