Eps8^tm1.2Kshsu
Targeted Allele Detail

Summary

• Symbol: Eps8^tm1.2Kshsu
• Name: epidermal growth factor receptor pathway substrate 8; targeted mutation 1.2, Kuei-Sen Hsu
• MGI ID: MGI:5817754
• Gene: Eps8 Location: Chr6:137454242-137626262 bp, - strand Genetic Position: Chr6, 66.78 cM
• Alliance: Eps8^tm1.2Kshsu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237769
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 was flanked by loxP sites and an FRT site flanked reporter-selection double cassette was inserted into intron 4. The double cassette consists of a beta galactosidase (lacZ) gene cassette separated from a neomycin resistance gene cassette by a loxP site. The lacZ-neo double cassette was removed through flp-mediated recombination. Exon 5 was deleted by cre-mediated recombination, creating a knock-out allele. RT-PCR confirmed lack of wild-type transcripts. (J:237769)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eps8 Mutation: 124 strains or lines available

References

• Original: J:237769 Wang YT, et al., Conditional deletion of Eps8 reduces hippocampal synaptic plasticity and impairs cognitive function. Neuropharmacology. 2017 Jan;112(Pt A):113-123
• All: 1 reference(s)