Esyt1^tm1Tmss
Targeted Allele Detail

Summary

• Symbol: Esyt1^tm1Tmss
• Name: extended synaptotagmin-like protein 1; targeted mutation 1, Tom Moss
• MGI ID: MGI:5817203
• Synonyms: esyt1^flox-neo
• Gene: Esyt1 Location: Chr10:128346117-128361728 bp, - strand Genetic Position: Chr10, 76.85 cM
• Alliance: Esyt1^tm1Tmss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236096
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeted KO-first, conditional ready, lacZ-tagged mutant vector PRPGS00088_C_F09 vector from the EUCOMM targeting project 69413 was used to insert an FRT site followed by, splice acceptor site, lacZ sequence with polyadenilation sequence, and a loxP site, followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site into an intron 2 of the gene. A third loxP site is inserted into intron 7. The critical exons 3 to 7 are thus flanked by loxP sites. (J:236096)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Esyt1 Mutation: 50 strains or lines available

References

• Original: J:236096 Tremblay MG, et al., Loss of all 3 Extended Synaptotagmins does not affect normal mouse development, viability or fertility. Cell Cycle. 2016 Sep;15(17):2360-6
• All: 1 reference(s)