Mki67^{tm2.1(cre/ERT2)Cle}
Targeted Allele Detail

Summary

• Symbol: Mki67^{tm2.1(cre/ERT2)Cle}
• Name: antigen identified by monoclonal antibody Ki 67; targeted mutation 2.1, Hans Clevers
• MGI ID: MGI:5816737
• Synonyms: Ki67^iresCreEr
• Gene: Mki67 Location: Chr7:135291513-135318090 bp, - strand Genetic Position: Chr7, 81.32 cM, cytoband F3-F5
• Alliance: Mki67^{tm2.1(cre/ERT2)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:254891
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Mki67^{tm2.1(cre/ERT2)Cle} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mki67 (MGI:106035)

• Mutation details: A targeting vector containing ires2-creERT2 and SV40 polyadenylation site fllowed by a FRT site flanked PGKneo cassette was used to introduce cre/ERT2 at the C-terminus of the Mki67 gene. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:254891)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mki67 (mouse) Summary of all recombinase alleles driven by Mki67. Alliance mouse Mki67 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mki67 Mutation: 147 strains or lines available

References

• Original: J:254891 Basak O, et al., Troy+ brain stem cells cycle through quiescence and regulate their number by sensing niche occupancy. Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):E610-E619
• All: 7 reference(s)