Nova1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Nova1^{tm1c(EUCOMM)Hmgu}
• Name: NOVA alternative splicing regulator 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5812231
• Synonyms: Nova1^LoxP
• Gene: Nova1 Location: Chr12:46744678-46866143 bp, - strand Genetic Position: Chr12, 21.46 cM
• Alliance: Nova1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Nova1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236975
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 46767266 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 46768069. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:236975)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nova1 Mutation: 21 strains or lines available

References

• Original: J:236975 Vernia S, et al., An alternative splicing program promotes adipose tissue thermogenesis. Elife. 2016 Sep 16;5:e17672
• All: 1 reference(s)