Dpy30tm1d(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Dpy30tm1d(KOMP)Wtsi |
Name: |
dpy-30, histone methyltransferase complex regulatory subunit; targeted mutation 1d, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5812162 |
Synonyms: |
Dpy30- |
Gene: |
Dpy30 Location: Chr17:74606469-74630939 bp, - strand Genetic Position: Chr17, 45.64 cM
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Alliance: |
Dpy30tm1d(KOMP)Wtsi page
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IMPC: |
Dpy30 gene page |
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Mutant Cell Line: |
EPD0714_4_A06 |
Germline Transmission: |
Earliest citation of germline transmission:
J:237262
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 74614423 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 74615334. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exons.
(J:237262)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Dpy30 Mutation: |
45 strains or lines available
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Original: |
J:237262 Yang Z, et al., Dpy30 is critical for maintaining the identity and function of adult hematopoietic stem cells. J Exp Med. 2016 Oct 17;213(11):2349-2364 |
All: |
3 reference(s) |
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