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Tg(Hsf4/EGFP)N7Spbh
Transgene Detail
Nomenclature
Symbol: Tg(Hsf4/EGFP)N7Spbh
Name: transgene insertion N7, Suraj P Bhat
MGI ID: MGI:5810681
Transgene: Tg(Hsf4/EGFP)N7Spbh  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Tg(Hsf4/EGFP)N7Spbh expresses 1 gene
 
Mutation detailsThis transgene consists of BAC clone RP24-227N21(170 kb) containing the mouse Hsf4 gene with enhanced green fluorescent coding sequence with a polyadenylation signal sequence inserted at the end of exon 1 of Hsf4 that codes for part of the DNA binding domain and a FRT-Tn5-neo-FRT cassette. Flpe-mediated recombination removed the Tn5-neo cassette. This BAC contains genes between Cbfb and Hspc171. The transgene produces the mRNA for the hybrid gene containing the exon 1 sequences of the Hsf4 gene and the EGFP coding sequences and the mRNA translates into a approximate 32 kDa hybrid protein (N-terminal 41 amino acids of Hsf4 and 239 amino acids EGFP). Four lines (N1, N4, N7 and N8) were generated. Line N7 is entered as a representative line with the most severe phenotype. (J:230205)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:230205 Gangalum RK, et al., Expression of the HSF4 DNA binding domain-EGFP hybrid gene recreates early childhood lamellar cataract in transgenic mice. Invest Ophthalmol Vis Sci. 2014 Nov;55(11):7227-40
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/16/2021
MGI 6.16
The Jackson Laboratory