Rasgrf2^{tm2.1(folA/flpo)Hze}
Targeted Allele Detail

Summary

• Symbol: Rasgrf2^{tm2.1(folA/flpo)Hze}
• Name: RAS protein-specific guanine nucleotide-releasing factor 2; targeted mutation 2.1, Hongkui Zeng
• MGI ID: MGI:5806617
• Synonyms: Rasgrf2-2A-dgFlpO-D, Rasgrf2-2A-dgFlpO-deltaneo
• Gene: Rasgrf2 Location: Chr13:92028519-92268164 bp, - strand Genetic Position: Chr13, 47.43 cM, cytoband C3-D1
• Alliance: Rasgrf2^{tm2.1(folA/flpo)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260362
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Rasgrf2^{tm2.1(folA/flpo)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Rasgrf2 (MGI:109137)

• Mutation details: The targeting vector contained, from 5' to 3': a partial Rasgrf2 sequence spanning intron 29-33 (with an FRT3 site in intron 33) and partial exon 34 sequence up to (but not including) the endogenous stop codon, a viral 2A oligopeptide sequence (T2A), an in-frame dgFlpO fusion gene (described below), a bovine growth hormone polyA sequence, an AttB site, a PGK-Neomycin cassette, an FRT5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene, and an AttP site. The dgFlpO fusion gene is an enhanced green fluorescent protein/FlpO recombinase fusion gene with an N terminal fusion of the first 159 amino acids of the Escherichia coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to also include the R12Y/Y100I destabilizing domain mutations. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL). (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rasgrf2 (mouse) Summary of all recombinase alleles driven by Rasgrf2. Alliance mouse Rasgrf2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rasgrf2 Mutation: 73 strains or lines available

References

• Original: J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22
• All: 3 reference(s)