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Rasgrf2tm2.1(folA/flpo)Hze
Targeted Allele Detail
Nomenclature
Symbol: Rasgrf2tm2.1(folA/flpo)Hze
Name: RAS protein-specific guanine nucleotide-releasing factor 2; targeted mutation 2.1, Hongkui Zeng
MGI ID: MGI:5806617
Synonyms: Rasgrf2-2A-dgFlpO-D, Rasgrf2-2A-dgFlpO-deltaneo
Gene: Rasgrf2  Location: Chr13:91880400-92131656 bp, - strand  Genetic Position: Chr13, 47.43 cM, cytoband C3-D1
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe targeting vector contained, from 5' to 3': a partial Rasgrf2 sequence spanning intron 29-33 (with an FRT3 site in intron 33) and partial exon 34 sequence up to (but not including) the endogenous stop codon, a viral 2A oligopeptide sequence (T2A), an in-frame dgFlpO fusion gene (described below),a bovine growth hormone polyA sequence, an AttB site, a PGK-Neomycin cassette, an FRT5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene, and an AttP site. The dgFlpO fusion gene is an enhanced green fluorescent protein/FlpO recombinase fusion gene with an N terminal fusion of the first 159 amino acids of the Escherichia coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to also include the R12Y/Y100I destabilizing domain mutations. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL). (J:101977)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Rasgrf2     Summary of all recombinase alleles driven by Rasgrf2.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rasgrf2 Mutation:  3 strains or lines available
References
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/22/2020
MGI 6.16
The Jackson Laboratory