Peg3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Peg3^{tm1c(EUCOMM)Hmgu}
• Name: paternally expressed 3; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5806481
• Synonyms: Flip KO
• Gene: Peg3 Location: Chr7:6706891-6733430 bp, - strand Genetic Position: Chr7, 3.89 cM, cytoband A2-B1
• Alliance: Peg3^{tm1c(EUCOMM)Hmgu} page
• IMPC: Peg3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232917
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 6718777 of Chromosome 7 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 6719585. Exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, deleting the lacZ sequence and neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:232917)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Peg3 Mutation: 59 strains or lines available

References

• Original: J:232917 Perera BP, et al., Yy1 gene dosage effect and bi-allelic expression of Peg3. PLoS One. 2015;10(3):e0119493
• All: 3 reference(s)