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Tg(Mt1-TGFBR2*)AM3Epb
Transgene Detail
Nomenclature
Symbol: Tg(Mt1-TGFBR2*)AM3Epb
Name: transgene insertion AM3, Erwin P Bottinger
MGI ID: MGI:5806465
Synonyms: DNR
Transgene: Tg(Mt1-TGFBR2*)AM3Epb  Location: unknown  
Transgene
origin
Strain of Origin:  FVB/N
Transgene
description
Transgene Type:    Transgenic (Dominant negative, Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Mt1-TGFBR2*)AM3Epb expresses 1 gene
 
Mutation detailsThe human sequence between nucleotides -7 to 573, encoding extracellular and transmembrane domains followed by a stop codon at the 3' end, is under the control of the mouse metallothionein 1 promoter and locus control regions. Activity of the promoter is induced with zinc. This results in the expression of a dominant-negative mutant receptor. Northern blot analysis confirmed RNA expression. Two lines were generated, AM3 and BF1. Line AM3 carries three copies per haploid genome and shows highest zinc-induced RNA expression in the pancreas, liver, colon and small intestine. Substantial levels of the dominant negative receptor is expressed in the liver and pancreas without zinc induction. Immunohistochemistry confirmed protein cell-surface expression in acinar cells in the pancreas and hepatocytes in the liver. (J:76993)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:76993 Bottinger EP, et al., Expression of a dominant-negative mutant TGF-beta type II receptor in transgenic mice reveals essential roles for TGF-beta in regulation of growth and differentiation in the exocrine pancreas. EMBO J. 1997 May 15;16(10):2621-33
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/22/2020
MGI 6.16
The Jackson Laboratory