Zranb1^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zranb1^{tm1d(EUCOMM)Hmgu}
• Name: zinc finger, RAN-binding domain containing 1; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5806463
• Synonyms: Zranb1 KO
• Gene: Zranb1 Location: Chr7:132532905-132588127 bp, + strand Genetic Position: Chr7, 76.32 cM
• Alliance: Zranb1^{tm1d(EUCOMM)Hmgu} page
• IMPC: Zranb1 gene page

Mutation origin

• Mutant Cell Line: HEPD0538_8_D07
• Germline Transmission: Earliest citation of germline transmission: J:235701
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 132567698 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 132568709. A knockout allele was created by flp and cre recombinase expression in mice, removing the lacZ sequence, the neomycin selection cassette and the critical exons. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:235701)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zranb1 Mutation: 48 strains or lines available

References

• Original: J:235701 Jin J, et al., Epigenetic regulation of the expression of Il12 and Il23 and autoimmune inflammation by the deubiquitinase Trabid. Nat Immunol. 2016 Mar;17(3):259-68
• All: 1 reference(s)