Cdh4^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cdh4^{tm1c(EUCOMM)Wtsi}
• Name: cadherin 4; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5806242
• Synonyms: Cdh4^flox
• Gene: Cdh4 Location: Chr2:179084228-179541166 bp, + strand Genetic Position: Chr2, 101.86 cM
• Alliance: Cdh4^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cdh4 gene page

Mutation origin

• Mutant Cell Line: EPD0336_1_D06
• Germline Transmission: Earliest citation of germline transmission: J:235675
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 179086004 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 179086835. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ and neomycin sequences. The critical exon(s) is/are thus flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:235675)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdh4 Mutation: 55 strains or lines available

References

• Original: J:235675 Martinez-Garay I, et al., Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex. Development. 2016 Jun 15;143(12):2121-34
• All: 1 reference(s)