Avil^{tm1(HBEGF)Phep}
Targeted Allele Detail

Summary

• Symbol: Avil^{tm1(HBEGF)Phep}
• Name: advillin; targeted mutation 1, Paul A Heppenstall
• MGI ID: MGI:5805490
• Synonyms: Avil^iDTR, Avil-iDTR
• Gene: Avil Location: Chr10:126836578-126856863 bp, + strand Genetic Position: Chr10, 74.48 cM, cytoband D3
• Alliance: Avil^{tm1(HBEGF)Phep} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234423
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Avil^{tm1(HBEGF)Phep} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  monkey	HBEGF

• Mutation details: Exon 2 of the sensory neuron-specific Avril gene and 66 bp of DNA upstream thereof have been replaced by the Cre recombinase-inducible "iDTR cassette," which contains a loxP-flanked transcriptional/translational STOP cassette blocking expression of the open reading frame of the simian heparin binding EGF like growth factor (HBEGF) cDNA; unlike the mouse protein, primate HBEGF is a diphtheria toxin receptor (DTR). The loxP-flanked segment contains two SV40 polyadenylation signals p[A], a frt-flanked neo cassette, and the STOP cassette (comprising 550 bp of 3' sequence from the S. cerevisiae His3 gene, an SV40 p[A], and a sequence containing a false translation initiation codon (ATG) immediately followed by a splice donor sequence). Deletion of the STOP cassette by Cre recombinase allows expression of HBEGF under control of the endogenous Avil promoter, which renders Avil-expressing sensory neurons susceptible to ablation by diphtheria toxin. Immunohistochemical analysis of dorsal root ganglion (DRG) sections from mice bearing this allele in the absence of Cre using goat anti-human HB-EGF antibody detected no expression of DTR. In mice also expressing Cre recombinase in somatostatin-positive neurons, DTR was detected in a small subset of IB4^- DRG neurons; no signal was seen in sections taken from mice following DT administration. (J:118365, J:131076, J:234423)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Avil Mutation: 46 strains or lines available

References

• Original: J:234423 Stantcheva KK, et al., A subpopulation of itch-sensing neurons marked by Ret and somatostatin expression. EMBO Rep. 2016 Apr;17(4):585-600
• All: 10 reference(s)