Dll1^tm11.1Gos
Targeted Allele Detail

Summary

• Symbol: Dll1^tm11.1Gos
• Name: delta like canonical Notch ligand 1; targeted mutation 11.1, Achim Gossler
• MGI ID: MGI:5805263
• Synonyms: Dll1EGF3m, Dll1^EGF3m, Dll1^{tm3(Dll1*)Gos}
• Gene: Dll1 Location: Chr17:15587616-15597134 bp, - strand Genetic Position: Chr17, 8.95 cM
• Alliance: Dll1^tm11.1Gos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:230819
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: The transcribed region of the endogenous gene was replaced with a Dll1 minigene derived from a cDNA fragment encompassing exon 1 through the 5' part of exon 9 joined to a genomic DNA fragment containing the remainder of exon 9 through the polyadenylation signal, followed by a reverse-oriented, loxP-flanked neomycin selection cassette. Before the vector was used for gene targeting, the fourth and fifth cysteine codons (TGT or TGC) of the third EGF repeat (EGF3) were replaced by glycine codons (GGT or GGC) to prevent formation of the disulfide bridge. The neo cassette was later deleted in vivo by Cre recombinase. (J:230819)
• Inheritance: Recessive

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dll1 Mutation: 46 strains or lines available

Notes

The unnamed ES cell line used for targeting was generated from 129S1/SvImJ embryos in the laboratory of Dr. Achim Gossler.

References

• Original: J:230819 Schuster-Gossler K, et al., Context-Dependent Sensitivity to Mutations Disrupting the Structural Integrity of Individual EGF Repeats in the Mouse Notch Ligand DLL1. Genetics. 2016 Mar;202(3):1119-33
• All: 1 reference(s)