Tg(Prnp-PRNP)361Jmto
Transgene Detail

Summary

• Symbol: Tg(Prnp-PRNP)361Jmto
• Name: transgene insertion 361, Juan Maria Torres
• MGI ID: MGI:5803836
• Synonyms: tg(moPrpn 129Val-HuPrP-361) Jmtorres, tgVal129/tg361
• Transgene: Tg(Prnp-PRNP)361Jmto Location: unknown
• Alliance: Tg(Prnp-PRNP)361Jmto page

Transgene origin

• Strain of Origin: 129P2/OlaHsd or C57BL/6 or CBA

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp-PRNP)361Jmto expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PRNP (5621)

  Prnp	(MGI:97769)

  Val 129 variant

• Mutation details: Mice with this transgene express the Val129 variant of human prion protein under control of the mouse prion protein regulatory sequences. The transgene was generated by substitution of the open reading frame (ORF) of the human prion protein gene for the ORF in the third exon of the orthologous mouse gene in the expression vector MoPrP.Xho. This vector contains the mouse genomic sequence including exon 1, intron 1, and exon 2 joined directly to exon 3, in which the ORF has been replaced by an Xho1 restriction endonuclease site, thus providing the promoter, 5'-untranslated region (5'-UTR), and 3'-UTR from the mouse locus. Expression of the transgene-encoded protein in brains of homozygous transgenic mice is ~4-fold that in human brain, as determined by densitometric immunoblot analysis of serially diluted brain homogenates using the 3F4 monoclonal antibody, which recognizes the 109MKHM112 epitope of the human prion protein. (J:225676, J:235784)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

"The transgene was microinjected into pronuclear stage ova collected from B6CBAF1 females mated with 129/Ola males carrying a null mutation in endogenous Prp." (J:235784): (C57BL/6 x CBA)F1 x 129P2/OlaHsd-Prnp

References

• Original: J:225676 Cassard H, et al., Evidence for zoonotic potential of ovine scrapie prions. Nat Commun. 2014;5:5821
• All: 3 reference(s)