Tg(Tcra,Tcrb)562Biat
Transgene Detail

Summary

• Symbol: Tg(Tcra,Tcrb)562Biat
• Name: transgene insertion 562, Biomodels Austria
• MGI ID: MGI:5800328
• Synonyms: TCR-M
• Transgene: Tg(Tcra,Tcrb)562Biat Location: unknown
• Alliance: Tg(Tcra,Tcrb)562Biat page

Transgene origin

• Strain of Origin: BALB/c or C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tcra,Tcrb)562Biat expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)	binds cardiac myosin heavy chain alpha a.a. 614-629
  mouse	Tcrb (MGI:98578)

• Mutation details: A T cell hybridoma was generated using effector T helper (Th) cells from BALB/cAnNCrl mice affected by experimental autoimmune myocarditis (EAM) induced by a peptide corresponding to amino acids 614-629 of cardiac myosin heavy chain alpha. The variable region coding sequences of its T cell receptor alpha (Tcra: Va2-Ja42) and beta (Tcrb: Vb8-Db1-Jb2-4) mRNAs were cloned into Tcra and Tcrb expression cassettes, respectively. Each of these cassettes provides all the necessary 5' promoter sequences, the transcripional initiation site, and most of the 5' untranslated region of the mRNA residing upstream of a genomic V-region gene segment; the essential 3' enhancer sequences present upstream of the constant- (C-) region gene segment(s); and a C-region gene segment. The linearized constructs were co-injected into fertilized oocytes. Cell sorting followed by RT-PCR analysis showed that the transgene-encoded T cell receptor (TCR) Valpha2 and Vbeta8 chains are expressed in 80 to >95% of peripheral CD4⁺ T cells and almost all thymic CD4 single-positive (SP) T cells of transgenic mice. (J:99452, J:187943)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

"Linearized transgene constructs were injected at an equimolar ratio into fertilized oocytes of the (BALB/c x C57BL/6)F1 x BALB/c background." (J:187943)

References

• Original: J:187943 Nindl V, et al., Cooperation of Th1 and Th17 cells determines transition from autoimmune myocarditis to dilated cardiomyopathy. Eur J Immunol. 2012 Sep;42(9):2311-21
• All: 4 reference(s)