Vax1^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Vax1^{tm1c(KOMP)Mbp}
• Name: ventral anterior homeobox 1; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:5796178
• Synonyms: Vax1^flox
• Gene: Vax1 Location: Chr19:59154619-59158488 bp, - strand Genetic Position: Chr19, 54.61 cM
• Alliance: Vax1^{tm1c(KOMP)Mbp} page
• IMPC: Vax1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231756
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 59154265 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 59157472. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. The critical exon(s) is/are thus flanked by loxP sites. (J:231756)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vax1 Mutation: 14 strains or lines available

References

• Original: J:231756 Hoffmann HM, et al., Deletion of Vax1 from Gonadotropin-Releasing Hormone (GnRH) Neurons Abolishes GnRH Expression and Leads to Hypogonadism and Infertility. J Neurosci. 2016 Mar 23;36(12):3506-18
• All: 6 reference(s)