Traf3ip3^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Traf3ip3^{tm1b(KOMP)Wtsi}
• Name: TRAF3 interacting protein 3; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5795737
• Gene: Traf3ip3 Location: Chr1:192857812-192883854 bp, - strand Genetic Position: Chr1, 97.62 cM
• Alliance: Traf3ip3^{tm1b(KOMP)Wtsi} page
• IMPC: Traf3ip3 gene page

Mutation origin

• Mutant Cell Line: EPD0093_2_B04
• Germline Transmission: Earliest citation of germline transmission: J:226431
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 192879748 of Chromosome 1 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 192880831. The critical exon 3 is thus flanked by loxP sites. Cre expression without flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis confirmed the absence of protein expression in thymocytes. (J:226431)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Traf3ip3 Mutation: 32 strains or lines available

References

• Original: J:226431 Zou Q, et al., T cell development involves TRAF3IP3-mediated ERK signaling in the Golgi. J Exp Med. 2015 Jul 27;212(8):1323-36
• All: 2 reference(s)