Fto^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Fto^{tm1b(EUCOMM)Wtsi}
• Name: FTO alpha-ketoglutarate dependent dioxygenase; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5795627
• Gene: Fto Location: Chr8:92040153-92395067 bp, + strand Genetic Position: Chr8, 44.34 cM
• Alliance: Fto^{tm1b(EUCOMM)Wtsi} page
• IMPC: Fto gene page

Mutation origin

• Mutant Cell Line: EPD0103_5_A10
• Germline Transmission: Earliest citation of germline transmission: J:228836
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 92135427 of Chromosome 8 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 3 at position 92137108. The critical exon 3 is thus flanked by loxP sites. Cre expression without flp expression creates a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:228836)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fto Mutation: 71 strains or lines available

References

• Original: J:228836 Wang CY, et al., FTO modulates circadian rhythms and inhibits the CLOCK-BMAL1-induced transcription. Biochem Biophys Res Commun. 2015 Aug 28;464(3):826-32
• All: 3 reference(s)