Poglut1^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Poglut1^{tm1d(EUCOMM)Hmgu}
• Name: protein O-glucosyltransferase 1; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5792952
• Synonyms: Poglut1^delta
• Gene: Poglut1 Location: Chr16:38345499-38370620 bp, - strand Genetic Position: Chr16, 26.86 cM
• Alliance: Poglut1^{tm1d(EUCOMM)Hmgu} page
• IMPC: Poglut1 gene page

Mutation origin

• Mutant Cell Line: HEPD0700_1_A09
• Germline Transmission: Earliest citation of germline transmission: J:227336
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 38365885 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 38366666. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice. Exon 4 is flanked by loxP sites, with the lacZ sequences and neomycin selection cassette deleted. Cre-mediated recombination resulted in the deletion of exon 4 in this allele. (J:227336)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Poglut1 Mutation: 61 strains or lines available

References

• Original: J:227336 Ramkumar N, et al., Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2. PLoS Genet. 2015 Oct;11(10):e1005551
• All: 1 reference(s)