Shank3^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Shank3^{tm1c(KOMP)Mbp}
• Name: SH3 and multiple ankyrin repeat domains 3; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:5792940
• Gene: Shank3 Location: Chr15:89383826-89444464 bp, + strand Genetic Position: Chr15, 44.96 cM
• Alliance: Shank3^{tm1c(KOMP)Mbp} page
• IMPC: Shank3 gene page

Mutation origin

• Mutant Cell Line: DEPD00516_3_A07
• Germline Transmission: Earliest citation of germline transmission: J:233405
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 89389112 of Chromosome 15 upstream of the critical exon 9 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 9 at position 89389949. The critical exon 9 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:233405)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shank3 Mutation: 87 strains or lines available

References

• Original: J:233405 Lee J, et al., Shank3-mutant mice lacking exon 9 show altered excitation/inhibition balance, enhanced rearing, and spatial memory deficit. Front Cell Neurosci. 2015;9:94
• All: 1 reference(s)